non psrc family py416 Search Results


psrc  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc psrc
Fig. 2. Effects of anastrozole <t>and</t> <t>Src</t> inhibitor AZD0530 on signaling and cell cycle regulators. Cells were grown without estrogen in 5% charcoal-stripped fetal bovine serum supplemented with androstendione (no drug) and treated with no additional drug, AZD0530 (SI), anastrozole (Ana), or both (Ana + SI) for 48 h and then assayed for (A) <t>pSrc,</t> Src, pAkt, Akt, pMAPK, and MAPK levels and (B) p27, cyclin E, cyclin D1, Cdk2, Cdk4, and β-actin levels. C, cyclin E was immunoprecipitated and complexes were resolved and immunoblotted for associated proteins. D, cyclin E immunoprecipitates were assayed for associated kinase activity as per Materials and Methods. Radioactivity in histone H1 substrate was quantitated. Mean ± SE % maximum kinase activity from four repeat assays.
Psrc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-human polyclonal psrc-family kinase (psfk) py416  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc rabbit anti-human polyclonal psrc-family kinase (psfk) py416
Reduced protein expression and TCR signaling due to LCK C465R. A LCK and GAPDH immunoblots of whole cell lysates of patient or healthy control (HC) T-cell blasts unstimulated or stimulated with anti-CD3 (2 min), anti-CD3/CD28 (2 min), PMA/ionomycin (P/I, 5 min) or treated with the selective LCK-inhibitor A770041 (A77, 10 min). Orange arrowheads indicate LCK bands. B pZAP70, pSFK <t>(pY416),</t> pERK1/2 or GAPDH immunoblots, conditions as in A . Red arrowheads indicate pLCK bands, black arrowheads pFYN-T. Numbers below pERK1/2 blot indicate fold change pERK1/2 band intensity (HC unstimulated =1), intensity normalized to GAPDH loading (numbers below GAPDH blot; GAPDH signal intensity normalized to HC unstimulated) C LCK, HA-tag and GAPDH immunoblots of whole cell lysates of Jurkat E6.1, J.CaM1.6, and stable transduced cells line expressing either LCK WT or LCK C465R with or without on C-terminal HA-tag or transduced with pCDH containing only the expression cassette for the extracellular domain of the low-affinity nerve growth factor receptor (LNGFR) (J.CaM EV). D Overlay of Ca 2+ -flux measurement in the indicated cell lines. Experiment representative of 3 independent experiments. E pZAP70, pSFK (pY416), pERK1/2 immunoblots in J.CaM1.6 cells transduced with pLJM1 containing LCK WT or LCK C465R and stimulated with either anti-CD3 (2, 5 or 15 min), anti-CD3/CD28 (2 or 5 min), PMA/ionomycin (5 min) or left untreated
Rabbit Anti Human Polyclonal Psrc Family Kinase (Psfk) Py416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-human polyclonal psrc-family kinase (psfk) py416/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
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non psrc family py416  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc non psrc family py416
Reduced protein expression and TCR signaling due to LCK C465R. A LCK and GAPDH immunoblots of whole cell lysates of patient or healthy control (HC) T-cell blasts unstimulated or stimulated with anti-CD3 (2 min), anti-CD3/CD28 (2 min), PMA/ionomycin (P/I, 5 min) or treated with the selective LCK-inhibitor A770041 (A77, 10 min). Orange arrowheads indicate LCK bands. B pZAP70, pSFK <t>(pY416),</t> pERK1/2 or GAPDH immunoblots, conditions as in A . Red arrowheads indicate pLCK bands, black arrowheads pFYN-T. Numbers below pERK1/2 blot indicate fold change pERK1/2 band intensity (HC unstimulated =1), intensity normalized to GAPDH loading (numbers below GAPDH blot; GAPDH signal intensity normalized to HC unstimulated) C LCK, HA-tag and GAPDH immunoblots of whole cell lysates of Jurkat E6.1, J.CaM1.6, and stable transduced cells line expressing either LCK WT or LCK C465R with or without on C-terminal HA-tag or transduced with pCDH containing only the expression cassette for the extracellular domain of the low-affinity nerve growth factor receptor (LNGFR) (J.CaM EV). D Overlay of Ca 2+ -flux measurement in the indicated cell lines. Experiment representative of 3 independent experiments. E pZAP70, pSFK (pY416), pERK1/2 immunoblots in J.CaM1.6 cells transduced with pLJM1 containing LCK WT or LCK C465R and stimulated with either anti-CD3 (2, 5 or 15 min), anti-CD3/CD28 (2 or 5 min), PMA/ionomycin (5 min) or left untreated
Non Psrc Family Py416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
non psrc family py416 - by Bioz Stars, 2026-02
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psrc(py416), phospho src family kinases antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc psrc(py416), phospho src family kinases antibody
Reduced protein expression and TCR signaling due to LCK C465R. A LCK and GAPDH immunoblots of whole cell lysates of patient or healthy control (HC) T-cell blasts unstimulated or stimulated with anti-CD3 (2 min), anti-CD3/CD28 (2 min), PMA/ionomycin (P/I, 5 min) or treated with the selective LCK-inhibitor A770041 (A77, 10 min). Orange arrowheads indicate LCK bands. B pZAP70, pSFK <t>(pY416),</t> pERK1/2 or GAPDH immunoblots, conditions as in A . Red arrowheads indicate pLCK bands, black arrowheads pFYN-T. Numbers below pERK1/2 blot indicate fold change pERK1/2 band intensity (HC unstimulated =1), intensity normalized to GAPDH loading (numbers below GAPDH blot; GAPDH signal intensity normalized to HC unstimulated) C LCK, HA-tag and GAPDH immunoblots of whole cell lysates of Jurkat E6.1, J.CaM1.6, and stable transduced cells line expressing either LCK WT or LCK C465R with or without on C-terminal HA-tag or transduced with pCDH containing only the expression cassette for the extracellular domain of the low-affinity nerve growth factor receptor (LNGFR) (J.CaM EV). D Overlay of Ca 2+ -flux measurement in the indicated cell lines. Experiment representative of 3 independent experiments. E pZAP70, pSFK (pY416), pERK1/2 immunoblots in J.CaM1.6 cells transduced with pLJM1 containing LCK WT or LCK C465R and stimulated with either anti-CD3 (2, 5 or 15 min), anti-CD3/CD28 (2 or 5 min), PMA/ionomycin (5 min) or left untreated
Psrc(Py416), Phospho Src Family Kinases Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psrc(py416), phospho src family kinases antibody/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
psrc(py416), phospho src family kinases antibody - by Bioz Stars, 2026-02
90/100 stars
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psrc family py416  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc psrc family py416
Reduced protein expression and TCR signaling due to LCK C465R. A LCK and GAPDH immunoblots of whole cell lysates of patient or healthy control (HC) T-cell blasts unstimulated or stimulated with anti-CD3 (2 min), anti-CD3/CD28 (2 min), PMA/ionomycin (P/I, 5 min) or treated with the selective LCK-inhibitor A770041 (A77, 10 min). Orange arrowheads indicate LCK bands. B pZAP70, pSFK <t>(pY416),</t> pERK1/2 or GAPDH immunoblots, conditions as in A . Red arrowheads indicate pLCK bands, black arrowheads pFYN-T. Numbers below pERK1/2 blot indicate fold change pERK1/2 band intensity (HC unstimulated =1), intensity normalized to GAPDH loading (numbers below GAPDH blot; GAPDH signal intensity normalized to HC unstimulated) C LCK, HA-tag and GAPDH immunoblots of whole cell lysates of Jurkat E6.1, J.CaM1.6, and stable transduced cells line expressing either LCK WT or LCK C465R with or without on C-terminal HA-tag or transduced with pCDH containing only the expression cassette for the extracellular domain of the low-affinity nerve growth factor receptor (LNGFR) (J.CaM EV). D Overlay of Ca 2+ -flux measurement in the indicated cell lines. Experiment representative of 3 independent experiments. E pZAP70, pSFK (pY416), pERK1/2 immunoblots in J.CaM1.6 cells transduced with pLJM1 containing LCK WT or LCK C465R and stimulated with either anti-CD3 (2, 5 or 15 min), anti-CD3/CD28 (2 or 5 min), PMA/ionomycin (5 min) or left untreated
Psrc Family Py416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psrc family py416/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
psrc family py416 - by Bioz Stars, 2026-02
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caveolin-1 antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc caveolin-1 antibody
Reduced protein expression and TCR signaling due to LCK C465R. A LCK and GAPDH immunoblots of whole cell lysates of patient or healthy control (HC) T-cell blasts unstimulated or stimulated with anti-CD3 (2 min), anti-CD3/CD28 (2 min), PMA/ionomycin (P/I, 5 min) or treated with the selective LCK-inhibitor A770041 (A77, 10 min). Orange arrowheads indicate LCK bands. B pZAP70, pSFK <t>(pY416),</t> pERK1/2 or GAPDH immunoblots, conditions as in A . Red arrowheads indicate pLCK bands, black arrowheads pFYN-T. Numbers below pERK1/2 blot indicate fold change pERK1/2 band intensity (HC unstimulated =1), intensity normalized to GAPDH loading (numbers below GAPDH blot; GAPDH signal intensity normalized to HC unstimulated) C LCK, HA-tag and GAPDH immunoblots of whole cell lysates of Jurkat E6.1, J.CaM1.6, and stable transduced cells line expressing either LCK WT or LCK C465R with or without on C-terminal HA-tag or transduced with pCDH containing only the expression cassette for the extracellular domain of the low-affinity nerve growth factor receptor (LNGFR) (J.CaM EV). D Overlay of Ca 2+ -flux measurement in the indicated cell lines. Experiment representative of 3 independent experiments. E pZAP70, pSFK (pY416), pERK1/2 immunoblots in J.CaM1.6 cells transduced with pLJM1 containing LCK WT or LCK C465R and stimulated with either anti-CD3 (2, 5 or 15 min), anti-CD3/CD28 (2 or 5 min), PMA/ionomycin (5 min) or left untreated
Caveolin 1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
caveolin-1 antibody - by Bioz Stars, 2026-02
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antilck  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc antilck
Reduced protein expression and TCR signaling due to LCK C465R. A LCK and GAPDH immunoblots of whole cell lysates of patient or healthy control (HC) T-cell blasts unstimulated or stimulated with anti-CD3 (2 min), anti-CD3/CD28 (2 min), PMA/ionomycin (P/I, 5 min) or treated with the selective LCK-inhibitor A770041 (A77, 10 min). Orange arrowheads indicate LCK bands. B pZAP70, pSFK <t>(pY416),</t> pERK1/2 or GAPDH immunoblots, conditions as in A . Red arrowheads indicate pLCK bands, black arrowheads pFYN-T. Numbers below pERK1/2 blot indicate fold change pERK1/2 band intensity (HC unstimulated =1), intensity normalized to GAPDH loading (numbers below GAPDH blot; GAPDH signal intensity normalized to HC unstimulated) C LCK, HA-tag and GAPDH immunoblots of whole cell lysates of Jurkat E6.1, J.CaM1.6, and stable transduced cells line expressing either LCK WT or LCK C465R with or without on C-terminal HA-tag or transduced with pCDH containing only the expression cassette for the extracellular domain of the low-affinity nerve growth factor receptor (LNGFR) (J.CaM EV). D Overlay of Ca 2+ -flux measurement in the indicated cell lines. Experiment representative of 3 independent experiments. E pZAP70, pSFK (pY416), pERK1/2 immunoblots in J.CaM1.6 cells transduced with pLJM1 containing LCK WT or LCK C465R and stimulated with either anti-CD3 (2, 5 or 15 min), anti-CD3/CD28 (2 or 5 min), PMA/ionomycin (5 min) or left untreated
Antilck, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antilck/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
antilck - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


Fig. 2. Effects of anastrozole and Src inhibitor AZD0530 on signaling and cell cycle regulators. Cells were grown without estrogen in 5% charcoal-stripped fetal bovine serum supplemented with androstendione (no drug) and treated with no additional drug, AZD0530 (SI), anastrozole (Ana), or both (Ana + SI) for 48 h and then assayed for (A) pSrc, Src, pAkt, Akt, pMAPK, and MAPK levels and (B) p27, cyclin E, cyclin D1, Cdk2, Cdk4, and β-actin levels. C, cyclin E was immunoprecipitated and complexes were resolved and immunoblotted for associated proteins. D, cyclin E immunoprecipitates were assayed for associated kinase activity as per Materials and Methods. Radioactivity in histone H1 substrate was quantitated. Mean ± SE % maximum kinase activity from four repeat assays.

Journal: Clinical Cancer Research

Article Title: Combined Src and Aromatase Inhibition Impairs Human Breast Cancer Growth In vivo and Bypass Pathways Are Activated in AZD0530-Resistant Tumors

doi: 10.1158/1078-0432.ccr-08-3127

Figure Lengend Snippet: Fig. 2. Effects of anastrozole and Src inhibitor AZD0530 on signaling and cell cycle regulators. Cells were grown without estrogen in 5% charcoal-stripped fetal bovine serum supplemented with androstendione (no drug) and treated with no additional drug, AZD0530 (SI), anastrozole (Ana), or both (Ana + SI) for 48 h and then assayed for (A) pSrc, Src, pAkt, Akt, pMAPK, and MAPK levels and (B) p27, cyclin E, cyclin D1, Cdk2, Cdk4, and β-actin levels. C, cyclin E was immunoprecipitated and complexes were resolved and immunoblotted for associated proteins. D, cyclin E immunoprecipitates were assayed for associated kinase activity as per Materials and Methods. Radioactivity in histone H1 substrate was quantitated. Mean ± SE % maximum kinase activity from four repeat assays.

Article Snippet: Western analysis of cyclin E, p27, Cdk2, Cdk4, cyclin D1, MAPK, pMAPK (MAPKpT202/Y204), Akt, pAkt (AktpT473), Src, and pSrc (Src-pY416) used the following: antibodies to MAPK, pMAPK, Akt, pAkt, Src, and pSrc were obtained from Cell Signaling; antibodies to p27 were from Transduction Laboratories; antibodies to cyclin E1 (monoclonal antibodies E172 and E12) were from E. Harlow (MGH); and antibodies to β-actin were from Sigma.

Techniques: Immunoprecipitation, Activity Assay, Radioactivity

Reduced protein expression and TCR signaling due to LCK C465R. A LCK and GAPDH immunoblots of whole cell lysates of patient or healthy control (HC) T-cell blasts unstimulated or stimulated with anti-CD3 (2 min), anti-CD3/CD28 (2 min), PMA/ionomycin (P/I, 5 min) or treated with the selective LCK-inhibitor A770041 (A77, 10 min). Orange arrowheads indicate LCK bands. B pZAP70, pSFK (pY416), pERK1/2 or GAPDH immunoblots, conditions as in A . Red arrowheads indicate pLCK bands, black arrowheads pFYN-T. Numbers below pERK1/2 blot indicate fold change pERK1/2 band intensity (HC unstimulated =1), intensity normalized to GAPDH loading (numbers below GAPDH blot; GAPDH signal intensity normalized to HC unstimulated) C LCK, HA-tag and GAPDH immunoblots of whole cell lysates of Jurkat E6.1, J.CaM1.6, and stable transduced cells line expressing either LCK WT or LCK C465R with or without on C-terminal HA-tag or transduced with pCDH containing only the expression cassette for the extracellular domain of the low-affinity nerve growth factor receptor (LNGFR) (J.CaM EV). D Overlay of Ca 2+ -flux measurement in the indicated cell lines. Experiment representative of 3 independent experiments. E pZAP70, pSFK (pY416), pERK1/2 immunoblots in J.CaM1.6 cells transduced with pLJM1 containing LCK WT or LCK C465R and stimulated with either anti-CD3 (2, 5 or 15 min), anti-CD3/CD28 (2 or 5 min), PMA/ionomycin (5 min) or left untreated

Journal: Journal of Clinical Immunology

Article Title: A Novel Biallelic LCK Variant Resulting in Profound T-Cell Immune Deficiency and Review of the Literature

doi: 10.1007/s10875-023-01602-8

Figure Lengend Snippet: Reduced protein expression and TCR signaling due to LCK C465R. A LCK and GAPDH immunoblots of whole cell lysates of patient or healthy control (HC) T-cell blasts unstimulated or stimulated with anti-CD3 (2 min), anti-CD3/CD28 (2 min), PMA/ionomycin (P/I, 5 min) or treated with the selective LCK-inhibitor A770041 (A77, 10 min). Orange arrowheads indicate LCK bands. B pZAP70, pSFK (pY416), pERK1/2 or GAPDH immunoblots, conditions as in A . Red arrowheads indicate pLCK bands, black arrowheads pFYN-T. Numbers below pERK1/2 blot indicate fold change pERK1/2 band intensity (HC unstimulated =1), intensity normalized to GAPDH loading (numbers below GAPDH blot; GAPDH signal intensity normalized to HC unstimulated) C LCK, HA-tag and GAPDH immunoblots of whole cell lysates of Jurkat E6.1, J.CaM1.6, and stable transduced cells line expressing either LCK WT or LCK C465R with or without on C-terminal HA-tag or transduced with pCDH containing only the expression cassette for the extracellular domain of the low-affinity nerve growth factor receptor (LNGFR) (J.CaM EV). D Overlay of Ca 2+ -flux measurement in the indicated cell lines. Experiment representative of 3 independent experiments. E pZAP70, pSFK (pY416), pERK1/2 immunoblots in J.CaM1.6 cells transduced with pLJM1 containing LCK WT or LCK C465R and stimulated with either anti-CD3 (2, 5 or 15 min), anti-CD3/CD28 (2 or 5 min), PMA/ionomycin (5 min) or left untreated

Article Snippet: After a blocking step with 3% BSA TBS-T, immunoblotting was performed with the following antibodies: mouse anti-human FYN (clone E-3), mouse anti-human GAPDH (clone 6C5), mouse anti-human LCK (clone 3A5), mouse-IgGκ BP-HRP, rabbit-IgGκ BP-HRP, anti-mouse-IgG-HRP (all Santa Cruz Biotechnology) or rabbit anti-human pZAP70 pY319 (clone 65E4), rabbit anti-human polyclonal pSRC-family kinase (pSFK) pY416, rabbit anti-human pERK1/2 pT202/pY204 (clone 197G2), and rabbit anti-HA-tag (clone C29F4) (all Cell Signaling Technologies).

Techniques: Expressing, Western Blot, Control, Transduction